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Inventi Impact: Gene Medicines

Articles

  • Inventi:pgm/31395/20
    A Generic Method for Fast and Sensitive Detection of Adeno-Associated Viruses Using Modified AAV\nReceptor Recombinant Proteins
    Research 2020 : April - June
    Mengtian Cui, Yabin Lu, Can Tang, Ran Zhang, Jing Wang, Yang Si, Shan Cheng, Wei Ding

    Adeno-Associated Viruses (AAV) are widely used gene-therapy vectors for both clinical\napplications and laboratory investigations. The titering of different AAV preparations is important\nfor quality control purposes, as well as in comparative studies. However, currently available methods\nare limited in their ability to detect various serotypes with sensitivity and convenience. Here, we took\nadvantage of a newly discovered AAV receptor protein with high affinity to multiple AAV serotypes,\nand developed an ELISA-like method named â??VIRELISAâ? (virus receptor-linked immunosorbent\nassay) by adopting fusion with a streptavidin-binding peptide (SBP). It was demonstrated that\noptimized VIRELISA assays exhibited satisfactory performance for the titering of AAV2â?¦..â?¦â?¦â?¦

    How to Cite this Article
    Attribution/ CC Compliant Citation: Cui, Mengtian, et al. \"A Generic Method for Fast and Sensitive Detection of Adeno-\nAssociated Viruses Using Modified AAV Receptor Recombinant Proteins.\" Molecules 24.21 (2019): 3973.\nhttps://doi:10.3390/molecules24213973\nhttp://creativecommons.org/licenses/by/4.0/\nSome formatting elements, header, footer, logos, dates and pagination were modified while adapting this article.
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